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By V. Z. Gorkin

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This hypo­ thesis is supported by the data (Bond and Cundall, 1977) on the inhibition by semicarbazide of the deamination of benzylamine by a suspension of granulo­ cytes. This inhibitory effect was observed at lower concentrations of semi­ carbazide than in similar systems using thrombocytes or lymphocytes. Increased sensitivity towards carbonyl reagents, accompanied by decreased sensitivity to the effects of monoamine oxidase inhibitors, is character­ istic of chemically modified (owing to partial oxidation of -SH groups) purified monoamine oxidases and for membrane-bound mitochondrial monoamine oxidases, which have undergone qualitative reversible alteration (trans­ formation) in their catalytic activity (Gorkin, 1973, 1976).

1 mM dithiothreitol was completely solubilized by treatment with 1% Triton X-100 for 30 min. The precipitate obtained after centrifugation (60 min at 100,000 g) was extracted twice under the same conditions and the supernatants were combined f (total volume 87 ml) (Kandaswami and D l o r i o , 1978). Inactivation of monoamine oxidases was observed in the presence of high concentrations (2% or more) or Triton X-100 and/or prolonged (24 hours or longer) treatment of membranes with the detergent (Yasunobu and coworkers, 1968).

8 Μ NaClO^ in the presence of 3-mercaptoethanol for 30 min at 0°C and centrifuged (10 min at 105,000 g). Only 10-20% of rat liver mito­ chondrial monoamine oxidase activity and 25% of human liver mitochondrial monoamine oxidase activity could be solubilized using this technique. As shown by inhibitor analysis, solubilized rat or human liver monoamine oxidases exhibited the properties of type Β monoamine oxidases only. Combined treatment with low concentrations of urea and Triton X-100 resulted in solubilization of 70-90% of the monoamine oxidase activity (substrate serotonin, 3-phenylethylamine and dopamine) from bovine brainstem mitochon­ dria without any alteration in the ratio of the deamination rates of the various amines characteristic of the original mitochondria (Moskvitina and coworkers, 1979).

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